Tritium

Our associated TRITIUM LABELING Lab from the Russian Academy of Sciences in Moscow, Russia is highly qualified, very specialized and ready to handle any known tritium labeling procedures and/or techniques.  Among the methods and/or approaches used are:                                                                                                                                       

·         Catalytic reduction with tritium gas – specific labeling

·         Catalytic dehalogenation with tritium gas – specific labeling

·         Exchange reactions using tritium gas – random labeling

·         Exchange reactions using tritiated water – random labeling

·         Chemical and enzymatic reactions – specific and random labeling

Examples of Specific Procedures

·         High Temperature Solid state Catalytic Isotope Exchange (HTSCIE) with Tritium gas: the compound to be labeled is coated on a solid Pd or Pt catalyst, exposed to tritium gas at a given optimum temperature and pressure, then eluted and isolated via preparative HPLC.

·         Palladium Oxide Catalyzed Tritium Exchange (POCTE): a proprietary method similar to above.

·         Combination of above tritiation procedures of an appropriate chemical precursor to the desired final compound followed by the full chemical or enzymatic synthesis of the desired final product.

 Comments

·         We can label virtually any compound that has catalytically exchangeable or chemically replaceable hydrogen atoms, by choosing from the above methods the one that will better preserve the chemical integrity and the biological activity of the given compound.

·         The variety of the classes of compounds that we succeeded to label ranged from “small but reactive molecules” to Peptides, Proteins, Oligonucleotides, and Macrocyclic complex biologically active compounds. We were quite successful in labeling these complex molecules at high specific activities where our competitors could not succeed.

·         We also specialize in labeling Proprietary Compounds under the strict rules of Confidentiality Agreements required by our clients (for obvious reasons these compounds are not listed below).

·         When catalytic exchange reactions are to be used for highly confidential compounds, we do not need ask for the full disclosure of the structure of the compound. We would need to know the only the general characteristics of the molecule such as number of sulphur containing functional groups, S-S bridges, epoxy groups that could hinder the labeling or other reactive functional groups that might not withstand the tritiation reactions we might contemplate to apply.

·         All orders are custom made for a minimum amount of one mCi or more where applicable due to the low mass/high specific activity relationship for given compound. If needed, larger amounts of labeled material can also be prepared either by choosing different methods or via repetitive batches.

·         All products are being purified by preparative HPLC and finally analyzed using HPLC and other chromatographic methods.

·         Our products have a Chemical and Radiochemical Purity of at least 97% by HPLC or higher if you so desire.

·         Where applicable, our Lab could also custom synthesize medium size non labeled proprietary peptides containing unsaturated functional groups that upon saturation with tritium will lead to the desired specific tritium labeled compounds or to subject them to their tritiation via random labeling.

·         Enzymatic reactions have also been used for the labeling of some complex biologically active molecules, followed by the tedious preparative HPLC separation of the desired product from the complex reaction mixture (see the various alkyl Coenzyme A derivatives;  Rapamycine; Leukotriene B4, etc.).

ORDERING

Please check the List of Compounds from below for the names of all tritium labeled compounds that we MADE OVER THE PAST 15 YEARS as custom syntheses. These compounds were prepared as a result of specific requests from specific customers. Because of that and the limited stability upon storage of high specific activity tritium labeled organic molecules, the listed compounds are not available from stock and have to be synthesized each time anew in a minimum amount of one milliCurie.  Upon ordering, please specify the name of the compound; the desired amount, specific activity and purity; the HPLC system (if any) that you prefer and the shipping solvent desired.  If you do not find your compound below, please inquire with us by e-mail or phone.